Protein Assays
Promega ADP-Glo™ Max Assay
The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.
Promega Transfection Carrier DNA
A plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA.
Promega HiBiT Control Protein, 100μL
A 20μm control protein. Use as a positive control with the Nano-Glo HiBiT Detection and Blotting Systems.
Promega PDK1 Kinase Enzyme System
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
![Promega pNLCoI3[<i>luc2</i>-P2A-<i>NlucP</i>/CMV/Hygro] Vector](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRN1481.jpg-250.jpg)
Promega pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
Promega Nano-Glo HiBiT Lytic Detection System, 10 x 100ml
Accurately quantify Any HiBiT Tagged Protein Expressed in Cells in an antibody free, plate based method.
![Promega pNL1.1.PGK[<i>Nluc</i>/PGK] Vector](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRN1441.jpg-250.jpg)
Promega pNL1.1.PGK[Nluc/PGK] Vector
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System.
Promega pNL3.2.CMV Vector
NanoLuc (Nluc) luciferase is a small bright luminescent reporter enzyme (19.1kDa) engineered for optimal performance. Use the pNL3.2.CMV Vector as a negative control in experiments measuring regulated changes in NanoLuc luciferase expression.
Promega pFC32A Nluc CMV-Hygro Flexi™ Vector
Generate C-Terminal Fusions to NanoLuc™ Luciferase Reporter. The pFC32A and pFC32K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.
Promega Nano-Glo™ Dual-Luciferase™ Reporter Assay/pNL1.1.TK Bundle
The Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.
Promega pFN11A (BIND) Flexi™ Vector
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
Promega pFN31K Nluc CMV-neo Flexi™ Vector
Generate N-Terminal fusions to NanoLuc™ Luciferase Reporter. The pFN31A and pFN31K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.
![Promega pNLF1-N [CMV/Hygro] Vector](https://assets.fishersci.com/TFS-Assets/CCG/Promega-Corporation/product-images/FCATPRN1351.jpg-250.jpg)
Promega pNLF1-N [CMV/Hygro] Vector
Generate N-Terminal Fusions with NanoLuc™ Enzyme. The pNLF1 Vectors use traditional cloning with a multiple cloning site (MCS) to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.
![Promega pNLF1-secN [CMV/Hygro] Vector](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRN1371.jpg-250.jpg)
Promega pNLF1-secN [CMV/Hygro] Vector
Generate N-Terminal Fusions with N-Terminal Secreted NanoLuc™ Luciferase. The pNLF1 Vectors use traditional cloning with a multiple cloning site to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.
![Promega pNLCoI1[<i>luc2</i>-P2A-<i>Nluc</i>P/Hygro] Vector](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRN1461.jpg-250.jpg)
Promega pNLCoI1[luc2-P2A-NlucP/Hygro] Vector
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.